p53 ELISA Kit

Catalog No. ABIN5520904

This Colorimetric ELISA kit is designed for the quantitative measurement of Human p53. There is 1 publication available.

Quick Overview for p53 ELISA Kit (ABIN5520904)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details p53 ELISA Kit

Minimum Detection Limit: 78.125 pg/mL

Purpose: For quantitative detection of TP53 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of TP53. No significant cross-reactivity or interference between TP53 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between TP53 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 46.875 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for p53 ELISA Kit (ABIN5520904)

Yuniarti, Mustofa, Aryandono, Haryana et al.: "Synergistic Action of 1,2-Epoxy-3 (3- (3,4-dimethoxyphenyl)- 4H-1-benzopiyran-4-on) Propane with Doxorubicin and Cisplatin through Increasing of p53, TIMP-3, and MicroRNA-34a in Cervical Cancer Cell ..." in: Asian Pacific journal of cancer prevention : APJCP, Vol. 19, Issue 10, pp. 2955-2962, (2019) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: TP53

Background: TP53/BCC7/LFS1/TRP53/Antigen NY-CO-13/FLJ92943/LFS1TRP53/p53/p53 tumor suppressor/P53cellular tumor antigen p53/Phosphoprotein p53/transformation-related protein 53/tumor protein p53/Tumor suppressor p53

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect